Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

34. Nucleic Acids

Intro to Nucleic Acids

Organic Chemistry

34. Nucleic Acids

Intro to Nucleic Acids

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Multiple Choice

What is the function of dideoxynucleotides in Sanger DNA sequencing?

They terminate DNA strand elongation.

They enhance the speed of DNA polymerase.

They stabilize the DNA double helix.

They act as primers for DNA synthesis.

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Verified step by step guidance

Understand the basic principle of Sanger DNA sequencing: It is a method used to determine the nucleotide sequence of DNA by synthesizing complementary strands and identifying the incorporated bases.

Recognize the role of dideoxynucleotides (ddNTPs): These are modified nucleotides that lack the 3'-hydroxyl group necessary for forming phosphodiester bonds, which are essential for DNA strand elongation.

Learn how ddNTPs function in the sequencing process: When a ddNTP is incorporated into a growing DNA strand, it prevents the addition of any further nucleotides, effectively terminating the elongation of that strand.

Explore the practical application in sequencing: By using a mixture of normal deoxynucleotides and ddNTPs, each labeled with a different fluorescent dye, the termination of strands at various lengths allows for the determination of the DNA sequence through capillary electrophoresis.

Clarify the incorrect options: ddNTPs do not enhance the speed of DNA polymerase, stabilize the DNA double helix, or act as primers for DNA synthesis. Their primary function is to terminate DNA strand elongation during sequencing.