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Multiple Choice
cDNA is synthesized from which molecule using an enzyme known as what?
A
rRNA using RNA polymerase
B
mRNA using reverse transcriptase
C
DNA using helicase
D
tRNA using DNA polymerase
Verified step by step guidance
1
Understand the concept of cDNA: Complementary DNA (cDNA) is a form of DNA synthesized from a messenger RNA (mRNA) template in a process called reverse transcription.
Identify the enzyme involved: The enzyme responsible for synthesizing cDNA from an mRNA template is reverse transcriptase.
Differentiate between the options: Recognize that rRNA, tRNA, and DNA are not templates for cDNA synthesis in this context. The correct template is mRNA.
Clarify the role of reverse transcriptase: This enzyme catalyzes the formation of cDNA from an mRNA template, which is crucial in processes like cloning and gene expression studies.
Review the incorrect options: RNA polymerase synthesizes RNA from a DNA template, DNA polymerase synthesizes DNA from a DNA template, and helicase unwinds DNA strands. These do not apply to cDNA synthesis from mRNA.