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Multiple Choice
DNA fragments cut by restriction enzymes can form two types of ends. What are these ends called?
A
Pointed ends and flat ends
B
Cohesive ends and non-cohesive ends
C
Sticky ends and blunt ends
D
Sharp ends and dull ends
Verified step by step guidance
1
Understand that restriction enzymes are proteins used to cut DNA at specific sequences, which is crucial in molecular biology for cloning and DNA manipulation.
Recognize that when restriction enzymes cut DNA, they can produce two types of ends: sticky ends and blunt ends.
Sticky ends, also known as cohesive ends, have overhanging single-stranded DNA sequences at the end of the cut. These overhangs can easily pair with complementary sequences, facilitating the ligation of DNA fragments.
Blunt ends are straight cuts across both DNA strands, resulting in no overhangs. While they can be ligated, it is generally less efficient than sticky ends due to the lack of complementary overhangs.
In summary, the two types of ends formed by restriction enzyme cuts are sticky ends and blunt ends, which are important for different applications in genetic engineering and molecular cloning.