Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

1. A Review of General Chemistry

Intro to Organic Chemistry

Organic Chemistry

1. A Review of General Chemistry

Intro to Organic Chemistry

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Multiple Choice

In the context of creating cDNA libraries, what is the template used for synthesis?

tRNA

mRNA

DNA

rRNA

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Verified step by step guidance

Understand the concept of cDNA libraries: cDNA libraries are collections of complementary DNA (cDNA) sequences that are synthesized from messenger RNA (mRNA) templates. They are used to study gene expression and function.

Identify the role of mRNA: mRNA serves as the template for cDNA synthesis because it represents the expressed genes in a cell. This allows researchers to focus on the coding regions of the genome.

Differentiate between mRNA and other types of RNA: mRNA is distinct from tRNA and rRNA, which are involved in protein synthesis but do not carry the coding information for proteins. Therefore, they are not used as templates for cDNA synthesis.

Recognize the process of reverse transcription: In cDNA library creation, reverse transcriptase is used to convert mRNA into cDNA. This enzyme synthesizes a DNA strand complementary to the mRNA template.

Conclude the correct template: Based on the understanding of cDNA library creation, mRNA is the correct template used for synthesis, as it provides the necessary coding information for generating cDNA.