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Multiple Choice
In the context of organic chemistry reactions, what is a potential consequence of leaving a stain on a bacterial smear for too long (over-staining)?
A
The stain may cause the bacterial cells to lyse.
B
The stain may enhance the visibility of bacterial structures.
C
The stain may obscure the morphology of the bacteria.
D
The stain may lead to the formation of crystal structures on the smear.
Verified step by step guidance
1
Understand the purpose of staining in microbiology: Staining is used to enhance the contrast of bacterial cells under a microscope, making it easier to observe their morphology and structures.
Consider the effects of over-staining: When a stain is left on a bacterial smear for too long, it can lead to excessive coloration.
Analyze the potential consequences of excessive coloration: Over-staining can obscure the fine details of bacterial morphology, making it difficult to accurately identify and study the bacteria.
Evaluate other potential outcomes: While over-staining might enhance visibility, it can also lead to misinterpretation of bacterial structures due to the overwhelming color.
Conclude with the most likely consequence: The primary issue with over-staining is that it can obscure the morphology of the bacteria, hindering proper analysis and identification.