Multiple Choice
In the context of organic chemistry reactions, what is a potential consequence of leaving a stain on a bacterial smear for too long (over-staining)?
3. Acids and Bases
Organic Chemistry Reactions
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In the context of organic chemistry reactions, what do the dark bands in a chromatography sample typically represent?
A
The different components of the mixture being separated
B
The presence of impurities in the sample
C
The baseline of the chromatogram
D
The solvent front in the chromatography process
Verified step by step guidance1
Chromatography is a technique used to separate components of a mixture based on their different interactions with a stationary phase and a mobile phase.
In a chromatography sample, the dark bands typically represent the different components of the mixture being separated. Each band corresponds to a distinct compound that has traveled a certain distance along the stationary phase.
The separation occurs because different compounds have varying affinities for the stationary phase and the mobile phase, causing them to move at different rates.
The presence of impurities in the sample can also be indicated by additional bands that do not correspond to the expected components.
The baseline of the chromatogram is the initial line where the sample is applied, and the solvent front is the furthest point reached by the solvent. These are not represented by the dark bands, which are indicative of the separated components.
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