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Multiple Choice
Which of the following is the primary dye used in the Gram-staining technique?
A
Methylene blue
B
Safranin
C
Carbol fuchsin
D
Crystal violet
Verified step by step guidance
1
Understand the purpose of the Gram-staining technique: It is used to differentiate bacterial species into two groups based on the chemical and physical properties of their cell walls.
Identify the primary dye in the Gram-staining process: The primary dye is the first stain applied to the bacterial sample, which initially colors all cells.
Recall the steps of the Gram-staining procedure: The process involves applying a primary dye, a mordant, a decolorizer, and a counterstain.
Recognize that Crystal violet is the primary dye: In the Gram-staining technique, Crystal violet is used as the primary stain to color all bacterial cells purple initially.
Note the role of other dyes: Safranin is used as a counterstain, and Methylene blue and Carbol fuchsin are not used as primary dyes in this technique.