Multiple Choice
In the context of organic chemistry reactions, what is the role of safranin in the Gram staining process for Gram-positive cells?
3. Acids and Bases
Organic Chemistry Reactions
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In gel electrophoresis of organic compounds, why do larger molecules tend to remain closer to the wells where the sample was loaded?
A
Larger molecules are more soluble in the gel, preventing them from moving far.
B
Larger molecules have a higher charge-to-mass ratio, causing them to move slower.
C
Larger molecules are repelled by the electric field, keeping them near the wells.
D
Larger molecules experience more friction and resistance, slowing their movement through the gel.
Verified step by step guidance1
Understand the principle of gel electrophoresis: It is a technique used to separate molecules based on size and charge by applying an electric field to a gel matrix.
Recognize that the gel matrix acts as a sieve: Smaller molecules can move more easily through the pores of the gel, while larger molecules experience more resistance.
Consider the role of friction and resistance: Larger molecules encounter more friction as they try to move through the gel, which slows their progress compared to smaller molecules.
Analyze the charge-to-mass ratio: While charge can influence movement, in this context, the size and resulting friction are the primary factors affecting the movement of larger molecules.
Conclude that larger molecules remain closer to the wells: Due to increased friction and resistance, larger molecules do not travel as far as smaller ones, resulting in their accumulation near the starting point.
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