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Multiple Choice
Which of the following statements correctly describes a key difference between the ELISA and Western Blot tests in the context of detecting HIV?
A
Western Blot is a faster test compared to ELISA for initial HIV screening.
B
ELISA is more specific than Western Blot in confirming HIV infection.
C
ELISA is used to detect HIV RNA, whereas Western Blot detects HIV DNA.
D
ELISA detects the presence of HIV antibodies, while Western Blot confirms the presence of specific HIV proteins.
Verified step by step guidance
1
Understand the purpose of each test: ELISA (Enzyme-Linked Immunosorbent Assay) is primarily used for initial screening to detect the presence of antibodies against HIV in a patient's blood sample.
Recognize the role of Western Blot: This test is used as a confirmatory test after a positive ELISA result. It detects specific proteins associated with HIV, providing confirmation of the virus's presence.
Differentiate between antibodies and proteins: ELISA detects antibodies, which are the immune system's response to the virus, while Western Blot identifies specific viral proteins, confirming the virus itself.
Consider the specificity and sensitivity: ELISA is highly sensitive and is used for initial screening due to its ability to detect even low levels of antibodies. Western Blot, on the other hand, is more specific, confirming the presence of HIV by identifying specific proteins.
Summarize the key difference: ELISA is used to detect the presence of HIV antibodies, indicating exposure to the virus, while Western Blot confirms the presence of specific HIV proteins, verifying the infection.