Multiple Choice
Which of the following is a common method for measuring liquid reagents in organic chemistry reactions?
3. Acids and Bases
Organic Chemistry Reactions
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In the context of organic chemistry reactions, how are restriction enzymes used in the process of making recombinant proteins?
A
They act as catalysts in the polymerization of nucleotides.
B
They facilitate the folding of proteins into their active forms.
C
They cut DNA at specific sequences to allow insertion of foreign DNA.
D
They catalyze the formation of peptide bonds between amino acids.
Verified step by step guidance1
Restriction enzymes, also known as restriction endonucleases, are proteins that cut DNA at specific sequences, known as recognition sites. These sites are typically palindromic sequences in the DNA.
In the process of making recombinant proteins, restriction enzymes are used to cut both the plasmid DNA (vector) and the foreign DNA (gene of interest) at specific sites, creating compatible ends.
Once the DNA is cut, the foreign DNA can be inserted into the plasmid DNA. This is possible because the ends of the DNA fragments are complementary and can be joined together.
The enzyme DNA ligase is then used to seal the nicks in the sugar-phosphate backbone, creating a continuous DNA molecule that includes the foreign DNA.
The recombinant plasmid can then be introduced into a host cell, such as a bacterium, where it can be replicated and expressed, leading to the production of the recombinant protein.
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