Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

1. A Review of General Chemistry

Intro to Organic Chemistry

Organic Chemistry

1. A Review of General Chemistry

Intro to Organic Chemistry

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Multiple Choice

Why are next-generation DNA sequencing technologies referred to as sequencing-by-synthesis?

Because they rely on synthesizing RNA from DNA templates.

Because they use chemical synthesis to create artificial DNA sequences.

Because they break down DNA into individual nucleotides before sequencing.

Because they synthesize a complementary DNA strand while detecting the sequence of nucleotides.

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Verified step by step guidance

Understand the concept of sequencing-by-synthesis: This method involves synthesizing a new DNA strand complementary to the template strand while simultaneously detecting the sequence of nucleotides being added.

Recognize the role of DNA polymerase: In sequencing-by-synthesis, DNA polymerase is used to add nucleotides to the growing DNA strand, which is complementary to the template strand.

Identify the detection mechanism: As each nucleotide is incorporated into the new strand, a signal is generated (often through fluorescence or other detectable markers) that allows the identification of the specific nucleotide added.

Differentiate from other sequencing methods: Unlike methods that break down DNA into individual nucleotides or rely on synthesizing RNA, sequencing-by-synthesis focuses on real-time detection during the synthesis of a complementary strand.

Apply this understanding to the problem: The correct answer is that next-generation DNA sequencing technologies are referred to as sequencing-by-synthesis because they synthesize a complementary DNA strand while detecting the sequence of nucleotides.