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Multiple Choice
Which enzyme is responsible for forming covalent bonds between restriction fragments during DNA ligation?
A
DNA polymerase
B
Helicase
C
DNA ligase
D
RNA polymerase
Verified step by step guidance
1
Understand the role of each enzyme listed in the options: DNA polymerase, Helicase, DNA ligase, and RNA polymerase.
DNA polymerase is primarily involved in DNA replication, where it synthesizes new DNA strands by adding nucleotides to a pre-existing chain.
Helicase is responsible for unwinding the DNA double helix, separating the two strands to allow replication or transcription to occur.
RNA polymerase is involved in the synthesis of RNA from a DNA template during the process of transcription.
DNA ligase is the enzyme that forms covalent bonds between restriction fragments, specifically by joining the sugar-phosphate backbones of DNA strands, making it the correct answer for DNA ligation.