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Multiple Choice
During gel electrophoresis, how does DNA move through the gel?
A
DNA moves towards the negative electrode due to its positive charge.
B
DNA moves randomly due to thermal motion.
C
DNA moves towards the positive electrode due to its negative charge.
D
DNA remains stationary because it is neutral.
Verified step by step guidance
1
Understand the basic principle of gel electrophoresis: it is a technique used to separate DNA fragments based on size by applying an electric field.
Recognize that DNA molecules are negatively charged due to their phosphate backbone. This is a crucial point because it determines the direction of movement in an electric field.
In an electric field, negatively charged DNA will migrate towards the positive electrode (anode). This is because opposite charges attract.
Consider the structure of the gel, which acts as a sieve. Smaller DNA fragments move faster and further through the gel matrix than larger ones.
Eliminate incorrect options: DNA does not move towards the negative electrode, as it is negatively charged. It does not move randomly or remain stationary, as these do not align with the principles of electrophoresis.