Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

34. Nucleic Acids

Intro to Nucleic Acids

Organic Chemistry

34. Nucleic Acids

Intro to Nucleic Acids

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Multiple Choice

Why is DNA polymerase used in PCR heat-resistant?

It is derived from thermophilic organisms that thrive at high temperatures.

It is a synthetic enzyme designed specifically for PCR.

It is chemically modified to withstand high temperatures.

It is protected by a heat-resistant protein coat.

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Verified step by step guidance

Understand the role of DNA polymerase in PCR: DNA polymerase is an enzyme that synthesizes new strands of DNA complementary to the target sequence during the Polymerase Chain Reaction (PCR).

Recognize the conditions of PCR: PCR involves repeated cycles of heating and cooling, with the denaturation step requiring high temperatures (around 94-98°C) to separate the DNA strands.

Identify the need for heat resistance: Since PCR involves high temperatures, the DNA polymerase used must be able to withstand these conditions without denaturing.

Learn about thermophilic organisms: Thermophilic organisms are those that thrive in extremely hot environments, such as hot springs. Enzymes derived from these organisms, like Taq polymerase from Thermus aquaticus, are naturally heat-resistant.

Conclude why DNA polymerase is heat-resistant: The DNA polymerase used in PCR is derived from thermophilic organisms, which allows it to remain stable and functional at the high temperatures required for the denaturation step in PCR.