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Multiple Choice
What might be the result of not allowing a slide to air dry sufficiently prior to heat fixation in an organic chemistry reaction experiment?
A
The slide will become too brittle to handle.
B
The sample may be washed away during staining.
C
The slide will develop cracks due to uneven heating.
D
The sample will become more resistant to staining.
Verified step by step guidance
1
Understand the purpose of air drying: In organic chemistry experiments, especially those involving microscopy, air drying a slide before heat fixation is crucial to ensure that the sample adheres properly to the slide.
Consider the role of heat fixation: Heat fixation is used to kill the sample and fix it to the slide, making it easier to stain and observe under a microscope. If the slide is not dry, the heat can cause the sample to be washed away.
Analyze the impact of moisture: If the slide is not sufficiently air-dried, residual moisture can cause the sample to not adhere properly, leading to it being washed away during subsequent staining processes.
Evaluate the consequences of improper drying: Without proper adhesion, the sample may not be visible after staining, as it could be lost during the washing steps involved in the staining process.
Conclude the importance of drying: Ensuring the slide is completely dry before heat fixation is essential to maintain the integrity of the sample for accurate observation and analysis.