Multiple Choice
In the context of organic chemistry reactions, what does a zone of inhibition around a chemical-saturated disc indicate?
3. Acids and Bases
Organic Chemistry Reactions
Multiple Choice
In the context of organic chemistry reactions, what is the typical sequence of temperatures used in a PCR (Polymerase Chain Reaction) cycle?
A
Denaturation at 72°C, Annealing at 55°C, Extension at 95°C
B
Denaturation at 95°C, Annealing at 72°C, Extension at 55°C
C
Denaturation at 55°C, Annealing at 72°C, Extension at 95°C
D
Denaturation at 95°C, Annealing at 55°C, Extension at 72°C
Verified step by step guidance1
Understand the purpose of each step in the PCR cycle: Denaturation, Annealing, and Extension.
Denaturation: This step involves heating the reaction to a high temperature to separate the double-stranded DNA into single strands. Typically, this occurs at around 95°C.
Annealing: In this step, the temperature is lowered to allow primers to bind to the single-stranded DNA. This usually happens at a lower temperature, around 55°C.
Extension: During this phase, the temperature is adjusted to allow the DNA polymerase to synthesize a new DNA strand by adding nucleotides. This generally occurs at around 72°C.
Match the typical temperatures and steps to the options provided to identify the correct sequence: Denaturation at 95°C, Annealing at 55°C, Extension at 72°C.
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