Multiple Choice
Which type of organic reaction involves the loss of a small molecule from the reactant, resulting in the formation of a double bond?
3. Acids and Bases
Organic Chemistry Reactions
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Which of the following characteristics does not need to be standardized in a Kirby-Bauer assay?
A
Incubation temperature
B
Type of solvent used
C
Agar depth
D
Concentration of antibiotic
Verified step by step guidance1
Understand the purpose of the Kirby-Bauer assay, which is used to determine the susceptibility of bacteria to antibiotics by measuring the zone of inhibition around antibiotic disks on an agar plate.
Identify the factors that need to be standardized in the assay to ensure consistent and reliable results. These typically include incubation temperature, agar depth, and concentration of antibiotic, as they directly affect bacterial growth and the diffusion of the antibiotic.
Consider the role of incubation temperature, which must be standardized to ensure that bacterial growth conditions are consistent across all tests.
Examine the importance of agar depth, which affects the diffusion rate of the antibiotic and thus the size of the inhibition zone.
Recognize that the concentration of antibiotic must be standardized to ensure that the results are comparable across different tests. The type of solvent used is not typically standardized in this assay, as it does not directly impact the diffusion or activity of the antibiotic in the context of the Kirby-Bauer test.
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