Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

3. Acids and Bases

Acids and Bases

Organic Chemistry

3. Acids and Bases

Acids and Bases

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Multiple Choice

Which of the following is the ideal temperature for Taq polymerase function during PCR?

95°C

55°C

72°C

37°C

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Verified step by step guidance

Understand the role of Taq polymerase in PCR: Taq polymerase is a thermostable enzyme used to synthesize DNA during the polymerase chain reaction (PCR). It is derived from the bacterium Thermus aquaticus, which thrives in hot environments.

Identify the stages of PCR: PCR consists of three main stages - denaturation, annealing, and extension. Each stage requires different temperature settings to facilitate the reaction.

Recognize the temperature for each stage: Denaturation occurs at around 95°C, where the double-stranded DNA is separated into single strands. Annealing happens at a lower temperature, typically around 55°C, where primers bind to the single-stranded DNA. Extension, where Taq polymerase synthesizes new DNA strands, occurs at an optimal temperature for the enzyme.

Determine the optimal temperature for Taq polymerase: The ideal temperature for the extension phase, where Taq polymerase is most active, is around 72°C. This temperature allows the enzyme to efficiently add nucleotides to the growing DNA strand.

Conclude the ideal temperature for Taq polymerase function: Based on the understanding of PCR stages and enzyme activity, the optimal temperature for Taq polymerase during the extension phase is 72°C.