Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

1. A Review of General Chemistry

Intro to Organic Chemistry

Organic Chemistry

1. A Review of General Chemistry

Intro to Organic Chemistry

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Multiple Choice

Why is Taq DNA polymerase used in PCR reactions rather than a 'normal' DNA polymerase?

Taq DNA polymerase has a higher fidelity than normal DNA polymerases.

Taq DNA polymerase is resistant to the high temperatures used in PCR.

Taq DNA polymerase is more efficient at synthesizing DNA at room temperature.

Taq DNA polymerase can synthesize longer DNA strands than normal DNA polymerases.

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Verified step by step guidance

Understand the purpose of PCR (Polymerase Chain Reaction): PCR is a technique used to amplify specific DNA sequences, making millions of copies of a particular DNA segment.

Recognize the conditions of PCR: The process involves repeated cycles of heating and cooling, with temperatures reaching around 95°C to denature the DNA strands.

Identify the role of DNA polymerase in PCR: DNA polymerase is an enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand.

Consider the stability of enzymes at high temperatures: Most 'normal' DNA polymerases would denature and lose their function at the high temperatures used in PCR.

Learn about Taq DNA polymerase: Taq DNA polymerase is derived from the thermophilic bacterium Thermus aquaticus, which thrives in hot environments. This enzyme is heat-resistant and remains active during the high-temperature denaturation step of PCR, making it ideal for the process.