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Multiple Choice
In the context of the trp operon, what would be the effect of a mutation in the operator region?
A
The operon would be continuously expressed, regardless of tryptophan levels.
B
The operon would only be expressed in the presence of high tryptophan levels.
C
The operon would be unaffected by the mutation and function normally.
D
The operon would be permanently repressed, preventing any expression.
Verified step by step guidance
1
Understand the role of the trp operon: The trp operon is a group of genes that are used to produce tryptophan when it is not available in the environment. It is regulated by the presence of tryptophan, which acts as a corepressor.
Identify the function of the operator region: The operator is a segment of DNA where the repressor protein binds. When tryptophan levels are high, the repressor binds to the operator, preventing transcription of the operon.
Consider the effect of a mutation in the operator region: A mutation in the operator region could prevent the repressor from binding effectively, leading to continuous expression of the operon regardless of tryptophan levels.
Evaluate the possible outcomes: If the operator cannot bind the repressor, the operon would be continuously expressed. This is because the repressor cannot inhibit transcription without binding to the operator.
Conclude the impact of the mutation: The mutation in the operator region would likely result in the operon being continuously expressed, as the regulatory mechanism is disrupted.