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Multiple Choice
Which of the following types of solutions is most suitable for determining the solubility of a compound in a wet mount preparation?
A
Acidic solution
B
Non-polar solvent
C
Organic solvent
D
Aqueous solution
Verified step by step guidance
1
Understand the context: A wet mount preparation involves placing a sample in a liquid medium on a microscope slide. The goal is to observe the sample under a microscope, often in its natural state.
Consider the nature of solubility: Solubility is the ability of a substance to dissolve in a solvent. The choice of solvent affects how well the compound dissolves.
Evaluate the options: An acidic solution may alter the chemical structure of the compound. A non-polar solvent is typically used for non-polar compounds, which may not be suitable for all types of samples. An organic solvent might dissolve organic compounds but could also affect the sample's integrity.
Focus on the aqueous solution: An aqueous solution is water-based, which is generally compatible with biological samples and many compounds. It maintains the natural state of the sample without altering its structure significantly.
Conclude the reasoning: For a wet mount preparation, an aqueous solution is most suitable as it provides a neutral medium that is less likely to interfere with the sample's natural properties, making it ideal for observing solubility and maintaining sample integrity.