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Multiple Choice
Which of the following changes can denature an enzyme?
A
Increase in enzyme concentration
B
Decrease in substrate concentration
C
Increase in temperature
D
Addition of a non-polar solvent
Verified step by step guidance
1
Understand that denaturation of an enzyme involves the alteration of its three-dimensional structure, which can affect its functionality.
Recognize that an increase in enzyme concentration does not typically denature an enzyme, as it simply means more enzyme molecules are present.
A decrease in substrate concentration also does not denature an enzyme; it merely reduces the rate of reaction due to fewer substrate molecules available for the enzyme to act upon.
Identify that an increase in temperature can denature an enzyme because excessive heat can disrupt the hydrogen bonds and other interactions that maintain the enzyme's structure.
Realize that the addition of a non-polar solvent can denature an enzyme by disrupting hydrophobic interactions within the enzyme, leading to a loss of its native conformation.