Table of contents

3. Acids and Bases

Organic Chemistry Reactions

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Multiple Choice

In the context of polymerase chain reaction (PCR), what is the second step of a PCR cycle?

Denaturation

Annealing

Ligation

Extension

Verified step by step guidance

Understand that PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences.

Recognize that a PCR cycle consists of three main steps: Denaturation, Annealing, and Extension.

Identify that the first step, Denaturation, involves heating the DNA to separate its two strands.

Acknowledge that the second step is Annealing, where the temperature is lowered to allow primers to bind to the single-stranded DNA.

Note that the third step, Extension, involves DNA polymerase synthesizing a new DNA strand by adding nucleotides.

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