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Multiple Choice
Which organisms are commonly used to manufacture human insulin in biotechnology?
A
Bacillus subtilis
B
Escherichia coli
C
Saccharomyces cerevisiae
D
Pseudomonas aeruginosa
Verified step by step guidance
1
Understand the context: Biotechnology often uses microorganisms to produce substances like human insulin. This process involves genetic engineering to insert the human insulin gene into the microorganism's DNA.
Identify the common microorganisms used: The most commonly used organisms for manufacturing human insulin are Escherichia coli and Saccharomyces cerevisiae. These organisms are chosen due to their ability to express foreign genes efficiently.
Consider the characteristics of Escherichia coli: E. coli is a bacterium that is widely used in biotechnology because it grows quickly, is easy to manipulate genetically, and can produce large quantities of proteins.
Consider the characteristics of Saccharomyces cerevisiae: This yeast is also used in biotechnology for insulin production. It is favored for its ability to perform post-translational modifications similar to human cells, which is important for the proper functioning of insulin.
Evaluate the options: Bacillus subtilis and Pseudomonas aeruginosa are not commonly used for insulin production. Bacillus subtilis is used for other types of protein production, while Pseudomonas aeruginosa is not typically used due to pathogenicity concerns.