Multiple Choice
Which enzyme is required for the polymerase chain reaction (PCR) in organic chemistry reactions?
3. Acids and Bases
Organic Chemistry Reactions
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What is the primary function of Taq polymerase in a PCR reaction?
A
To provide a buffer solution
B
To synthesize new DNA strands
C
To denature the DNA strands
D
To act as a primer for DNA replication
Verified step by step guidance1
Understand the role of Taq polymerase in PCR: Taq polymerase is an enzyme used in the Polymerase Chain Reaction (PCR) to amplify DNA sequences.
Recognize the key function of Taq polymerase: Its primary function is to synthesize new DNA strands by adding nucleotides to a DNA template during the extension phase of PCR.
Identify the stages of PCR: PCR consists of three main stages - denaturation, annealing, and extension. Taq polymerase is active during the extension stage.
Clarify the process of DNA synthesis: During the extension phase, Taq polymerase extends the primers by adding complementary nucleotides to the single-stranded DNA template, effectively synthesizing new DNA strands.
Differentiate Taq polymerase from other components: Unlike primers or buffer solutions, Taq polymerase does not initiate replication or provide a chemical environment; it specifically catalyzes the formation of new DNA strands.
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