Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

3. Acids and Bases

Organic Chemistry Reactions

Organic Chemistry

3. Acids and Bases

Organic Chemistry Reactions

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Multiple Choice

Which of the following reagents is commonly used to detect the products of a polymerase chain reaction (PCR) in organic chemistry?

Sodium borohydride

Ethidium bromide

Lithium aluminum hydride

Grignard reagent

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Verified step by step guidance

Understand the context of the problem: Polymerase Chain Reaction (PCR) is a technique used to amplify DNA sequences. In organic chemistry, detecting the products of PCR often involves visualizing the DNA.

Recognize the role of ethidium bromide: Ethidium bromide is a fluorescent dye that intercalates between the bases of DNA. When exposed to ultraviolet light, it fluoresces, allowing visualization of DNA bands.

Consider the properties of the other reagents: Sodium borohydride, lithium aluminum hydride, and Grignard reagents are typically used in organic synthesis for reduction reactions and forming carbon-carbon bonds, not for DNA visualization.

Identify the application of ethidium bromide: In a laboratory setting, after running a gel electrophoresis to separate DNA fragments, ethidium bromide is used to stain the gel, making the DNA visible under UV light.

Conclude why ethidium bromide is the correct choice: Given its ability to bind to DNA and fluoresce, ethidium bromide is commonly used to detect and visualize the products of PCR, making it the appropriate reagent for this purpose.