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Multiple Choice
In the context of organic chemistry reactions, how is indole production typically detected?
A
By a green color change with Fehling's solution
B
By the formation of a yellow precipitate with iodine
C
By observing a blue color change with Benedict's solution
D
By using Kovac's reagent, which produces a red color
Verified step by step guidance
1
Understand the context: Indole production is often detected in microbiological assays, particularly in the identification of bacteria that can produce indole from tryptophan.
Identify the reagent used: Kovac's reagent is commonly used to detect indole production. It contains p-dimethylaminobenzaldehyde, which reacts with indole to produce a red color.
Recognize the chemical reaction: When indole is present, it reacts with the aldehyde group in Kovac's reagent, leading to the formation of a red compound. This color change is indicative of indole production.
Differentiate from other tests: Fehling's solution and Benedict's solution are used for detecting reducing sugars, not indole. The iodine test is used for starch detection, not indole.
Summarize the detection method: Indole production is confirmed by the appearance of a red color upon the addition of Kovac's reagent, which is specific for indole detection in organic chemistry and microbiology.