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Multiple Choice
cDNA is synthesized using which of the following as the starting material?
A
tRNA
B
mRNA
C
DNA
D
rRNA
Verified step by step guidance
1
Understand the concept of cDNA: Complementary DNA (cDNA) is synthesized from a messenger RNA (mRNA) template in a process called reverse transcription.
Identify the role of mRNA: mRNA serves as the template for synthesizing cDNA because it carries the genetic information from DNA that is translated into proteins.
Recognize the function of reverse transcriptase: This enzyme catalyzes the synthesis of cDNA from an mRNA template, highlighting the importance of mRNA in this process.
Differentiate between RNA types: tRNA (transfer RNA) and rRNA (ribosomal RNA) are not used for cDNA synthesis. tRNA is involved in protein synthesis, while rRNA is a component of ribosomes.
Conclude the starting material: Since cDNA is synthesized from mRNA, the correct starting material for cDNA synthesis is mRNA.