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Multiple Choice
Which of the following functional groups is present in the colored dyes used in the Gram staining technique?
A
Hydroxyl group
B
Amino group
C
Carboxyl group
D
Carbonyl group
Verified step by step guidance
1
Understand the context of the problem: Gram staining is a technique used in microbiology to differentiate bacterial species into two groups based on the chemical and physical properties of their cell walls.
Identify the role of dyes in Gram staining: The dyes used in this technique, such as crystal violet and safranin, interact with bacterial cell walls to produce a visible color change.
Recognize the functional groups in the dyes: The dyes typically contain functional groups that can form ionic or covalent bonds with the bacterial cell wall components.
Consider the properties of the amino group: Amino groups (\(\text{NH}_2\)) are known for their ability to form bonds with acidic components, which is crucial in the staining process.
Conclude that the amino group is the functional group present in the dyes used for Gram staining, as it facilitates the binding of the dye to the bacterial cell walls.