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Multiple Choice
In the context of acids and bases, why is the lacI gene expressed without the help of an activator like CRP?
A
The lacI gene expression is dependent on the presence of glucose.
B
The lacI gene is constitutively expressed, meaning it is always active without needing an activator.
C
The lacI gene is activated by the presence of lactose, which acts as an inducer.
D
The lacI gene requires a high pH environment to be expressed.
Verified step by step guidance
1
Understand the role of the lacI gene: The lacI gene encodes the lac repressor protein, which regulates the lac operon by binding to the operator region and preventing transcription in the absence of lactose.
Recognize the concept of constitutive expression: A gene that is constitutively expressed is always active, meaning it is transcribed continuously without the need for additional regulatory proteins or activators.
Identify the conditions affecting lacI expression: The lacI gene is not influenced by the presence of glucose or the pH environment. Instead, it is always expressed to produce the repressor protein.
Differentiate between the roles of inducers and activators: Lactose acts as an inducer by binding to the lac repressor, causing it to release from the operator and allowing transcription of the lac operon. This is different from an activator, which would enhance transcription directly.
Conclude that the lacI gene does not require an activator like CRP because it is constitutively expressed, ensuring the constant production of the lac repressor protein to regulate the lac operon.