Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

3. Acids and Bases

Organic Chemistry Reactions

Organic Chemistry

3. Acids and Bases

Organic Chemistry Reactions

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Multiple Choice

Which of the following statements accurately describes why Taq polymerase is used in PCR?

Taq polymerase is derived from human cells, making it ideal for PCR.

Taq polymerase can synthesize DNA in the absence of a template.

Taq polymerase is the only enzyme that can initiate DNA synthesis.

Taq polymerase is stable at the high temperatures used during PCR.

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Verified step by step guidance

Understand the role of Taq polymerase in PCR: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences. It requires a DNA polymerase enzyme to synthesize new DNA strands from a template.

Recognize the conditions of PCR: PCR involves repeated cycles of heating and cooling. The high temperatures are used to denature the DNA, separating the strands, which is essential for the synthesis of new DNA strands.

Identify the unique property of Taq polymerase: Taq polymerase is derived from the bacterium Thermus aquaticus, which thrives in hot environments. This enzyme is thermostable, meaning it remains active and stable at the high temperatures used in PCR.

Compare Taq polymerase with other DNA polymerases: Unlike many other DNA polymerases that would denature at high temperatures, Taq polymerase can withstand the heat, making it ideal for PCR where temperature cycling is necessary.

Conclude why Taq polymerase is used in PCR: The stability of Taq polymerase at high temperatures is crucial for the efficiency and success of PCR, as it allows the enzyme to function throughout the thermal cycles without denaturing.