Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

3. Acids and Bases

Organic Chemistry Reactions

Organic Chemistry

3. Acids and Bases

Organic Chemistry Reactions

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Multiple Choice

Which method is used to select and grow a resistant strain of E. coli in an organic chemistry experiment involving antibiotic resistance?

Introduce a plasmid with antibiotic resistance genes and grow on antibiotic-containing medium

Grow E. coli in a medium with high salt concentration

Use a nutrient-rich medium without antibiotics

Expose E. coli to UV light to induce mutations

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Verified step by step guidance

Understand the concept of antibiotic resistance: Antibiotic resistance in bacteria is often conferred by genes that can be introduced via plasmids. These plasmids are small, circular DNA molecules that can replicate independently of chromosomal DNA.

Identify the role of plasmids: In the context of selecting and growing a resistant strain of E. coli, plasmids carrying antibiotic resistance genes are introduced into the bacterial cells. This allows the bacteria to survive in environments containing the specific antibiotic.

Select the appropriate method: To select for E. coli that have successfully taken up the plasmid, the bacteria are grown on a medium that contains the antibiotic. Only those cells that have incorporated the plasmid with the resistance gene will survive and grow.

Consider the other options: Growing E. coli in a medium with high salt concentration or using a nutrient-rich medium without antibiotics does not select for antibiotic resistance. Exposing E. coli to UV light can induce mutations but is not a targeted method for selecting antibiotic resistance.

Conclude the selection process: By growing the transformed E. coli on an antibiotic-containing medium, you ensure that only the bacteria with the resistance plasmid survive, effectively selecting for the resistant strain.