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Multiple Choice
What provides the energy for DNA polymerization in a PCR reaction?
A
GTP
B
ATP
C
dNTPs
D
NADH
Verified step by step guidance
1
Understand the role of DNA polymerization in PCR: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences. DNA polymerization is the process of synthesizing a new DNA strand complementary to the template strand.
Identify the components involved in PCR: The key components include the DNA template, primers, DNA polymerase enzyme, and nucleotides (dNTPs).
Clarify the function of dNTPs: dNTPs (deoxynucleotide triphosphates) are the building blocks of DNA. They provide both the nucleotides needed for the new DNA strand and the energy required for the polymerization reaction.
Explain the energy source: During DNA polymerization, the energy is derived from the hydrolysis of the phosphate bonds in dNTPs. The breaking of these high-energy bonds releases energy that drives the formation of phosphodiester bonds between nucleotides.
Differentiate dNTPs from other molecules: While GTP, ATP, and NADH are energy carriers in various biological processes, they are not directly involved in providing energy for DNA polymerization in PCR. dNTPs are specifically used in this context.