Multiple Choice
What is one of the benefits of using recombinant DNA (rDNA) technology in organic chemistry?
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Intro to Organic Chemistry
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In the context of DNA replication, what is an Okazaki fragment?
A
A segment of RNA that initiates DNA synthesis.
B
A continuous strand of DNA synthesized on the leading strand.
C
A short segment of DNA synthesized on the lagging strand during DNA replication.
D
A protein that unwinds the DNA double helix.
Verified step by step guidance1
Understand the context of DNA replication: DNA replication is the process by which a DNA molecule is copied to produce two identical DNA molecules. It involves the unwinding of the double helix and the synthesis of new strands.
Identify the role of the leading and lagging strands: During DNA replication, the leading strand is synthesized continuously in the direction of the replication fork, while the lagging strand is synthesized discontinuously.
Define Okazaki fragments: Okazaki fragments are short segments of DNA that are synthesized on the lagging strand. They are created because the lagging strand is synthesized in short bursts, moving away from the replication fork.
Explain the synthesis process: On the lagging strand, DNA polymerase synthesizes Okazaki fragments in the 5' to 3' direction. Each fragment begins with a short RNA primer, which is later removed and replaced with DNA.
Clarify the role of DNA ligase: After the Okazaki fragments are synthesized, DNA ligase joins these fragments together to form a continuous DNA strand, completing the replication process on the lagging strand.
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