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Multiple Choice
In the context of organic chemistry, what is the medium used for diffusion in the Ouchterlony technique?
A
Polyacrylamide gel
B
Agarose gel
C
Silica gel
D
Cellulose acetate
Verified step by step guidance
1
Understand the Ouchterlony technique: It is a method used in immunology to detect the presence of specific antigens or antibodies through diffusion in a gel medium.
Identify the common gel mediums used in diffusion techniques: Agarose gel, polyacrylamide gel, silica gel, and cellulose acetate are all materials used in various laboratory techniques.
Recognize the properties of agarose gel: Agarose gel is a polysaccharide obtained from seaweed, commonly used in gel electrophoresis and immunodiffusion due to its porous nature, which allows for the diffusion of molecules.
Consider the application of agarose gel in immunodiffusion: In the Ouchterlony technique, agarose gel is preferred because it provides a stable medium for the diffusion of antigens and antibodies, facilitating the formation of visible precipitin lines.
Conclude the suitability of agarose gel for the Ouchterlony technique: Given its properties and common usage in immunodiffusion, agarose gel is the appropriate medium for the Ouchterlony technique.