Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

3. Acids and Bases

Organic Chemistry Reactions

Organic Chemistry

3. Acids and Bases

Organic Chemistry Reactions

Previous problemNext problem

Struggling with Organic Chemistry?

Join thousands of students who trust us to help them ace their exams!Watch the first video

Multiple Choice

What is the primary difference between PCR (Polymerase Chain Reaction) and RT-PCR (Reverse Transcription Polymerase Chain Reaction) in the context of organic chemistry reactions?

PCR is a qualitative technique, while RT-PCR is a quantitative technique.

PCR is used for protein synthesis, while RT-PCR is used for DNA synthesis.

PCR amplifies DNA, while RT-PCR amplifies RNA by converting it to DNA first.

PCR requires a reverse transcriptase enzyme, while RT-PCR does not.

Previous problemNext problem

Verified step by step guidance

Understand the basic concept of PCR: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences. It involves repeated cycles of denaturation, annealing, and extension to create multiple copies of a specific DNA segment.

Learn about RT-PCR: RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a variation of PCR that involves converting RNA into DNA before amplification. This is done using the enzyme reverse transcriptase, which synthesizes complementary DNA (cDNA) from an RNA template.

Identify the primary difference: The key difference between PCR and RT-PCR is the type of nucleic acid being amplified. PCR directly amplifies DNA, whereas RT-PCR first converts RNA into DNA and then amplifies the resulting cDNA.

Clarify the role of enzymes: In PCR, DNA polymerase is used to synthesize new DNA strands. In RT-PCR, reverse transcriptase is required to convert RNA into cDNA before the DNA polymerase can amplify the cDNA.

Consider the applications: PCR is typically used for amplifying DNA for various applications, such as cloning or sequencing. RT-PCR is used when the starting material is RNA, such as in gene expression studies or viral RNA detection.