Multiple Choice
Which type of reaction is most likely to occur when chemical #1 is introduced to an alkene?
3. Acids and Bases
Organic Chemistry Reactions
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What is the primary difference between PCR (Polymerase Chain Reaction) and RT-PCR (Reverse Transcription Polymerase Chain Reaction) in the context of organic chemistry reactions?
A
PCR is a qualitative technique, while RT-PCR is a quantitative technique.
B
PCR is used for protein synthesis, while RT-PCR is used for DNA synthesis.
C
PCR amplifies DNA, while RT-PCR amplifies RNA by converting it to DNA first.
D
PCR requires a reverse transcriptase enzyme, while RT-PCR does not.
Verified step by step guidance1
Understand the basic concept of PCR: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences. It involves repeated cycles of denaturation, annealing, and extension to create multiple copies of a specific DNA segment.
Learn about RT-PCR: RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a variation of PCR that involves converting RNA into DNA before amplification. This is done using the enzyme reverse transcriptase, which synthesizes complementary DNA (cDNA) from an RNA template.
Identify the primary difference: The key difference between PCR and RT-PCR is the type of nucleic acid being amplified. PCR directly amplifies DNA, whereas RT-PCR first converts RNA into DNA and then amplifies the resulting cDNA.
Clarify the role of enzymes: In PCR, DNA polymerase is used to synthesize new DNA strands. In RT-PCR, reverse transcriptase is required to convert RNA into cDNA before the DNA polymerase can amplify the cDNA.
Consider the applications: PCR is typically used for amplifying DNA for various applications, such as cloning or sequencing. RT-PCR is used when the starting material is RNA, such as in gene expression studies or viral RNA detection.
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