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Multiple Choice
Why does DNA move from the negative end of the gel to the positive end during gel electrophoresis?
A
Because DNA is hydrophobic and moves towards the water in the gel.
B
Because DNA is negatively charged and is attracted to the positive end.
C
Because DNA is positively charged and is attracted to the negative end.
D
Because DNA is neutral and moves randomly in the gel.
Verified step by step guidance
1
Understand the structure of DNA: DNA molecules have a backbone made of sugar and phosphate groups. The phosphate groups are negatively charged due to the presence of phosphate ions.
Recognize the principle of gel electrophoresis: This technique separates molecules based on size and charge by applying an electric field across a gel matrix.
Identify the charge of DNA: Since DNA has negatively charged phosphate groups, it will be attracted to the positive electrode (anode) in an electric field.
Consider the movement of DNA in the gel: When an electric field is applied, DNA will migrate from the negative electrode (cathode) towards the positive electrode (anode) due to its negative charge.
Conclude why DNA moves in this direction: The movement of DNA from the negative to the positive end during gel electrophoresis is due to its inherent negative charge, which is attracted to the positive end of the gel.