Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

34. Nucleic Acids

Intro to Nucleic Acids

Organic Chemistry

34. Nucleic Acids

Intro to Nucleic Acids

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Multiple Choice

Which of the following techniques is not based on nucleic-acid hybridization?

Northern blotting

Western blotting

Southern blotting

FISH (Fluorescence in situ hybridization)

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Verified step by step guidance

Understand the concept of nucleic-acid hybridization: It involves the pairing of complementary nucleic acid strands (DNA or RNA) to form a double-stranded structure. This is a fundamental principle used in various molecular biology techniques.

Identify techniques based on nucleic-acid hybridization: Techniques like Northern blotting, Southern blotting, and FISH (Fluorescence in situ hybridization) rely on nucleic-acid hybridization to detect specific sequences of DNA or RNA.

Northern blotting: This technique is used to study gene expression by detecting RNA (or mRNA) levels in a sample. It involves hybridization of a labeled probe to the RNA of interest.

Southern blotting: This technique is used to detect specific DNA sequences in a sample. It involves the transfer of DNA fragments to a membrane followed by hybridization with a labeled DNA probe.

Western blotting: Unlike the other techniques, Western blotting is used to detect proteins, not nucleic acids. It involves the use of antibodies to identify specific proteins, thus it is not based on nucleic-acid hybridization.