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Multiple Choice
Which of the following tools of DNA technology is incorrectly paired with its use?
A
DNA ligase - Joining DNA fragments together
B
Gel electrophoresis - Separating DNA fragments by size
C
Restriction enzymes - Cutting DNA at specific sequences
D
PCR - Sequencing DNA
Verified step by step guidance
1
Understand the role of each tool in DNA technology: DNA ligase, gel electrophoresis, restriction enzymes, and PCR.
DNA ligase is an enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It is correctly paired with joining DNA fragments.
Gel electrophoresis is a technique used to separate DNA fragments based on their size. DNA fragments are loaded into a gel and an electric current is applied, causing the fragments to move through the gel. Smaller fragments move faster than larger ones, allowing for separation by size. This is correctly paired.
Restriction enzymes are proteins that cut DNA at specific sequences, known as restriction sites. They are used to cut DNA into smaller fragments for analysis or recombination. This is correctly paired.
PCR (Polymerase Chain Reaction) is a technique used to amplify a specific segment of DNA, making millions of copies of a particular DNA sequence. It is not used for sequencing DNA, which involves determining the exact order of nucleotides in a DNA molecule. Therefore, PCR is incorrectly paired with sequencing DNA.