Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

34. Nucleic Acids

Intro to Nucleic Acids

Organic Chemistry

34. Nucleic Acids

Intro to Nucleic Acids

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Multiple Choice

Why is RNase used during DNA extraction?

To break down DNA into smaller fragments for easier analysis

To stabilize the DNA structure during the extraction process

To degrade RNA and prevent contamination of the DNA sample

To enhance the solubility of DNA in the extraction buffer

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Verified step by step guidance

Understand the role of RNase: RNase is an enzyme that specifically degrades RNA molecules. It is used during DNA extraction to ensure that RNA does not contaminate the DNA sample.

Recognize the importance of removing RNA: During DNA extraction, RNA can be present in the sample due to the cellular contents. If not removed, RNA can interfere with downstream applications and analyses of the DNA.

Learn about the extraction process: DNA extraction involves breaking open cells to release DNA, followed by purification steps to isolate DNA from other cellular components, including RNA.

Consider the consequences of RNA contamination: RNA contamination can affect the purity and concentration measurements of DNA, leading to inaccurate results in experiments such as PCR or sequencing.

Conclude the purpose of RNase: The primary purpose of RNase in DNA extraction is to degrade RNA, ensuring a pure DNA sample for further analysis and experimentation.