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Multiple Choice
In the context of organic chemistry reactions, when should the inoculating loop be sterilized in the Bunsen burner during a laboratory experiment?
A
After transferring a sample to prevent contamination of the sample.
B
Only at the beginning of the experiment to maintain sterility throughout.
C
Before transferring a sample to ensure no contamination.
D
It is not necessary to sterilize the loop in organic chemistry reactions.
Verified step by step guidance
1
Understand the purpose of sterilizing the inoculating loop: Sterilization is crucial to prevent contamination of samples during laboratory experiments.
Identify the correct timing for sterilization: The loop should be sterilized before transferring a sample to ensure that it is free from any contaminants that could affect the experiment.
Consider the context of organic chemistry: While sterilization is more commonly associated with microbiology, maintaining a clean environment is also important in organic chemistry to avoid introducing impurities.
Evaluate the options given: The option 'Before transferring a sample to ensure no contamination' aligns with the standard laboratory practice of sterilizing equipment before use.
Conclude with best practices: Always sterilize the inoculating loop before and after each use to maintain a contamination-free environment, even if the primary focus is on chemical reactions rather than biological samples.