Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

15. Analytical Techniques:IR, NMR, Mass Spect

Purpose of Analytical Techniques

Organic Chemistry

15. Analytical Techniques:IR, NMR, Mass Spect

Purpose of Analytical Techniques

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Multiple Choice

Why are polyacrylamide gels preferred over agarose gels for analyzing protein fractions?

Agarose gels are more suitable for analyzing nucleic acids rather than proteins.

Polyacrylamide gels are less expensive than agarose gels.

Agarose gels are more stable under high voltage conditions.

Polyacrylamide gels provide higher resolution for smaller molecules like proteins.

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Verified step by step guidance

Understand the purpose of using gels in electrophoresis: Gels are used to separate molecules based on size and charge. Proteins and nucleic acids are commonly analyzed using gel electrophoresis.

Recognize the difference in gel types: Agarose gels are typically used for larger molecules like nucleic acids, while polyacrylamide gels are used for smaller molecules like proteins.

Consider the resolution capability: Polyacrylamide gels have a tighter matrix compared to agarose gels, which allows for higher resolution and better separation of smaller molecules such as proteins.

Evaluate the stability and cost factors: While cost and stability under high voltage are considerations, the primary reason for choosing polyacrylamide gels for proteins is their ability to provide higher resolution.

Conclude with the main reason: Polyacrylamide gels are preferred for analyzing protein fractions because they offer higher resolution for smaller molecules, which is crucial for detailed protein analysis.