Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

1. A Review of General Chemistry

Intro to Organic Chemistry

Organic Chemistry

1. A Review of General Chemistry

Intro to Organic Chemistry

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Multiple Choice

In the context of PCR, what is the purpose of setting up a reaction without template DNA?

To enhance the specificity of the primers used

To optimize the annealing temperature of the reaction

To increase the yield of the desired DNA product

To serve as a negative control to detect contamination

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Verified step by step guidance

Understand the role of a negative control in PCR: A negative control is a reaction set up without template DNA to ensure that any amplification observed is due to the presence of the template and not due to contamination.

Recognize the importance of controls in experiments: Controls are essential in experiments to validate the results. In PCR, a negative control helps confirm that the reagents and environment are free from contaminating DNA.

Identify the components of a PCR reaction: A typical PCR reaction includes template DNA, primers, nucleotides, DNA polymerase, and buffer. In a negative control, the template DNA is omitted.

Consider the implications of contamination: If amplification occurs in the negative control, it indicates contamination, suggesting that the results of the PCR may not be reliable.

Conclude the purpose of the negative control: The primary purpose of setting up a PCR reaction without template DNA is to serve as a negative control to detect any contamination in the reagents or environment.