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Multiple Choice
In the context of organic chemistry reactions, when are chemical-saturated disks typically placed on a plate during a test of disinfectants?
A
Before the agar solidifies
B
During the pouring of the agar
C
After the agar has solidified
D
Before the agar is poured
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Verified step by step guidance
1
Understand the context: The problem is about testing disinfectants using chemical-saturated disks on an agar plate, a common method in microbiology to assess the effectiveness of disinfectants.
Identify the process: The agar plate method involves pouring a liquid agar medium into a petri dish and allowing it to solidify before applying any test substances.
Recognize the importance of solidification: The agar must be solidified to provide a stable surface for the chemical-saturated disks. This ensures that the disks remain in place and that the diffusion of the disinfectant into the agar is consistent.
Consider the timing: Placing the disks before the agar solidifies or during pouring would result in uneven distribution and potential sinking of the disks, affecting the accuracy of the test.
Conclude the correct step: Therefore, the chemical-saturated disks should be placed on the agar plate after the agar has completely solidified to ensure proper testing conditions.