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Multiple Choice
Which of the following statements correctly describes the difference between stains used for light microscopy and those used for electron microscopy in organic chemistry reactions?
A
Stains for light microscopy are used to enhance contrast by binding to nucleic acids, while stains for electron microscopy bind to lipids.
B
Stains for light microscopy are typically organic dyes, while stains for electron microscopy are heavy metal salts.
C
Stains for light microscopy are used to increase resolution, while stains for electron microscopy decrease resolution.
D
Stains for light microscopy are typically inorganic compounds, while stains for electron microscopy are organic dyes.
Verified step by step guidance
1
Understand the purpose of stains in microscopy: Stains are used to enhance contrast in microscopic images, making it easier to distinguish different structures or components within a sample.
Differentiate between light and electron microscopy: Light microscopy uses visible light to illuminate samples, while electron microscopy uses a beam of electrons, allowing for much higher resolution.
Identify the types of stains used in light microscopy: Light microscopy typically uses organic dyes that bind to specific cellular components, such as nucleic acids or proteins, to enhance contrast.
Identify the types of stains used in electron microscopy: Electron microscopy often uses heavy metal salts, such as lead or uranium, which scatter electrons and provide contrast by enhancing electron density differences in the sample.
Compare the statements: The correct statement is that stains for light microscopy are typically organic dyes, while stains for electron microscopy are heavy metal salts, as this accurately describes the materials used and their purpose in each type of microscopy.