Multiple Choice
In the context of organic chemistry, are all mutations in organic molecules harmful?
1. A Review of General Chemistry
Intro to Organic Chemistry
Struggling with Organic Chemistry?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
How does gel electrophoresis separate DNA fragments from each other?
A
By density, with denser fragments moving faster
B
By size, with smaller fragments moving faster through the gel
C
By shape, with more compact fragments moving slower
D
By charge, with positively charged fragments moving faster
Verified step by step guidance1
Understand the principle of gel electrophoresis: It is a technique used to separate DNA fragments based on their size. DNA molecules are negatively charged due to their phosphate backbone.
Prepare the gel: Typically, an agarose gel is used. The concentration of agarose can affect the resolution of separation, with higher concentrations better for smaller fragments.
Load the DNA samples into wells at one end of the gel. Apply an electric field across the gel, with the negative electrode (cathode) near the wells and the positive electrode (anode) at the opposite end.
As the electric field is applied, DNA fragments will migrate towards the positive electrode. Smaller fragments will move faster through the gel matrix than larger ones, due to less resistance.
Visualize the separated DNA fragments: After the electrophoresis run, the gel is stained with a DNA-binding dye, such as ethidium bromide, and viewed under UV light to see the bands representing different sized fragments.
2:18mWatch next
Master Organic molecules in your everyday life. with a bite sized video explanation from Johnny
Start learningRelated Videos
Related Practice
Intro to Organic Chemistry practice set
Problem sets built by lead tutorsExpert video explanations