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Multiple Choice
Which of the following statements regarding the Ames test is true?
A
The Ames test is a method to assess the mutagenic potential of chemical compounds.
B
The Ames test is used to determine the boiling point of organic compounds.
C
The Ames test is used to measure the acidity of organic acids.
D
The Ames test is a technique for synthesizing amino acids.
Verified step by step guidance
1
Understand the purpose of the Ames test: The Ames test is a biological assay to assess the mutagenic potential of chemical compounds. It is not related to physical properties like boiling points or acidity, nor is it a synthesis technique.
Identify the key components of the Ames test: It uses strains of the bacterium Salmonella typhimurium that carry mutations in genes involved in histidine synthesis. These strains cannot grow on a histidine-free medium unless a mutation occurs that reverses the effect.
Recognize the role of mutagens: A mutagen is an agent that causes genetic mutation. The Ames test evaluates whether a chemical can cause mutations in the DNA of the test organism, indicating its potential mutagenic properties.
Consider the experimental setup: The test involves exposing the bacteria to the chemical compound in question and observing the rate of mutation. An increased rate of mutation suggests that the compound is mutagenic.
Conclude with the correct statement: Based on the understanding of the Ames test, the true statement is that it is a method to assess the mutagenic potential of chemical compounds.