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Multiple Choice
Why does conjugation between an Hfr strain and an F- strain not result in two Hfr strains?
A
The F- strain does not receive the entire Hfr chromosome during conjugation.
B
The F- strain lacks the necessary enzymes to integrate the Hfr DNA.
C
The Hfr strain loses its fertility factor during the process.
D
Conjugation between Hfr and F- strains results in the formation of an F+ strain.
Verified step by step guidance
1
Understand the concept of Hfr and F- strains: Hfr (High frequency of recombination) strains have the F factor integrated into their chromosome, allowing them to transfer genetic material to F- strains during conjugation.
Recognize the process of bacterial conjugation: During conjugation, the Hfr strain attempts to transfer its entire chromosome to the F- strain through a pilus. However, this process is often interrupted before the entire chromosome is transferred.
Identify the limitations of the F- strain: The F- strain typically does not receive the entire Hfr chromosome, including the F factor, due to the interruption of the conjugation process.
Consider the role of enzymes: The F- strain lacks the necessary enzymes to integrate the partial Hfr DNA it receives into its own chromosome, preventing it from becoming an Hfr strain.
Conclude the outcome of the conjugation: Since the F- strain does not receive the complete F factor, it cannot become an Hfr strain. Instead, it may become an F+ strain if it receives enough of the F factor to form a plasmid.