Multiple Choice
Which of the following is not a primary site of action for antimicrobial drugs in bacteria?
3. Acids and Bases
Organic Chemistry Reactions
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In the context of organic chemistry reactions involving bacterial transformation, which type of agar plate is selective for transformed bacterial cells?
A
Nutrient agar plate
B
Blood agar plate
C
LB agar plate with antibiotic
D
LB agar plate
Verified step by step guidance1
Understand the context: In bacterial transformation, we introduce foreign DNA into bacteria, often to express a new trait such as antibiotic resistance.
Recognize the purpose of selective media: Selective agar plates are used to isolate and identify bacteria that have successfully taken up the foreign DNA and express the desired trait.
Identify the role of antibiotics: When bacteria are transformed with a plasmid containing an antibiotic resistance gene, only those that have successfully incorporated the plasmid will survive on agar plates containing the antibiotic.
Differentiate between types of agar plates: Nutrient agar and blood agar are non-selective and do not contain antibiotics, so they cannot distinguish transformed cells from non-transformed ones.
Conclude with the correct choice: LB agar plate with antibiotic is selective for transformed bacterial cells because it allows only those with the antibiotic resistance gene to grow.
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