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Multiple Choice
What color do gram-negative bacteria exhibit after Gram staining?
A
Blue
B
Pink
C
Purple
D
Green
Verified step by step guidance
1
Understand the Gram staining process: Gram staining is a method used to differentiate bacterial species into two groups based on the physical properties of their cell walls.
Learn about the cell wall structure: Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane.
Recognize the role of crystal violet: In Gram staining, crystal violet is the primary stain that colors all cells initially.
Identify the decolorization step: Alcohol or acetone is used to wash away the crystal violet from Gram-negative bacteria, which have a thinner peptidoglycan layer.
Understand the counterstain application: Safranin or fuchsine is applied as a counterstain, which colors Gram-negative bacteria pink, while Gram-positive bacteria remain purple.